PATagRFP-GRP78
(Plasmid
#238419)
-
PurposeExpresses GRP78 fused to the photoactivatable Tag-red fluorescent protein (PATagRFP-GRP78)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 238419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepEGFP-N1
- Backbone size w/o insert (bp) 3700
- Total vector size (bp) 9000
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name78-kDa glucose-regulated protein
-
Alt nameGRP78
-
Alt nameBiP
-
Alt nameHSPA5
-
SpeciesH. sapiens (human)
-
Insert Size (bp)4200
-
GenBank IDNM_005347.5
-
Entrez GeneHSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
-
Tag
/ Fusion Protein
- PATagRFP (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
PATagRFP-GRP78 was a gift from Amy Shiu Lee (Addgene plasmid # 238419 ; http://n2t.net/addgene:238419 ; RRID:Addgene_238419) -
For your References section:
GRP78 regulates CD44v membrane homeostasis and cell spreading in tamoxifen-resistant breast cancer. Tseng CC, Stanciauskas R, Zhang P, Woo D, Wu K, Kelly K, Gill PS, Yu M, Pinaud F, Lee AS. Life Sci Alliance. 2019 Aug 15;2(4):e201900377. doi: 10.26508/lsa.201900377. Print 2019 Aug. 10.26508/lsa.201900377 PubMed 31416894