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Addgene

PATagRFP-GRP78
(Plasmid #238419)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238419 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone size w/o insert (bp) 3700
  • Total vector size (bp) 9000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    78-kDa glucose-regulated protein
  • Alt name
    GRP78
  • Alt name
    BiP
  • Alt name
    HSPA5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • GenBank ID
    NM_005347.5
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
  • Tag / Fusion Protein
    • PATagRFP (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PATagRFP-GRP78 was a gift from Amy Shiu Lee (Addgene plasmid # 238419 ; http://n2t.net/addgene:238419 ; RRID:Addgene_238419)
  • For your References section:

    GRP78 regulates CD44v membrane homeostasis and cell spreading in tamoxifen-resistant breast cancer. Tseng CC, Stanciauskas R, Zhang P, Woo D, Wu K, Kelly K, Gill PS, Yu M, Pinaud F, Lee AS. Life Sci Alliance. 2019 Aug 15;2(4):e201900377. doi: 10.26508/lsa.201900377. Print 2019 Aug. 10.26508/lsa.201900377 PubMed 31416894