PBAN-tetOn-NKX3.1
(Plasmid
#238472)
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PurposeDoxycycline inducible expression of the NKX3.1 transcription factor for mural cell differentiation.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 238472 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonePBAN-PB-TRE3G
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNKX3.1
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SpeciesH. sapiens (human)
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Entrez GeneNKX3-1 (a.k.a. BAPX2, NKX3, NKX3.1, NKX3A)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PBAN-tetOn-NKX3.1 was a gift from Juan Melero-Martin (Addgene plasmid # 238472 ; http://n2t.net/addgene:238472 ; RRID:Addgene_238472) -
For your References section:
Robust differentiation of human pluripotent stem cells into mural progenitor cells via transient activation of NKX3.1. Lee U, Zhang Y, Zhu Y, Luo AC, Gong L, Tremmel DM, Kim Y, Villarreal VS, Wang X, Lin RZ, Cui M, Ma M, Yuan K, Wang K, Chen K, Melero-Martin JM. Nat Commun. 2024 Sep 30;15(1):8392. doi: 10.1038/s41467-024-52678-8. 10.1038/s41467-024-52678-8 PubMed 39349465