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Addgene

pMSCV-RUNX1-WT
(Plasmid #238536)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238536 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMSCV-F-BirA
  • Backbone size w/o insert (bp) 5202
  • Total vector size (bp) 7709
  • Modifications to backbone
    Added human RUNX1 driven by MSCV, and Puromycin driven by the PGK promoter
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RUNX1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1343
  • GenBank ID
    XM_054324894.1
  • Entrez Gene
    RUNX1 (a.k.a. AML1, AML1-EVI-1, AMLCR1, CBF2alpha, CBFA2, EVI-1, PEBP2aB, PEBP2alpha)
  • Promoter MSCV

Cloning Information for Gene/Insert 1

  • Cloning method Unknown
  • 5′ sequencing primer CTGAGCCCAGGCAAGATGAG
  • 3′ sequencing primer gagcatgcgctttagcagcc
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Puromycin
  • Insert Size (bp)
    600
  • GenBank ID
    ON783971.1
  • Promoter PGK

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer cggcattctgcacgcttcaa
  • 3′ sequencing primer aggggttgtgggctctttta
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-RUNX1-WT was a gift from Diane Krause (Addgene plasmid # 238536 ; http://n2t.net/addgene:238536 ; RRID:Addgene_238536)

  • For your References section:

    CDK9 phosphorylates RUNX1 to promote megakaryocytic fate in megakaryocytic-erythroid progenitors. Kwon N, Lu YC, Thompson EN, Mancuso RI, Wang L, Zhang PX, Krause DS. Blood. 2024 Oct 24;144(17):1800-1812. doi: 10.1182/blood.2024023963. 10.1182/blood.2024023963 PubMed 39102635
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