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Addgene

pLEX.(Cyto)iGlucoSnFR.H66A.L276V-mRuby2
(Plasmid #238943)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238943 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    iGlucoSnFR.L276V.K312A
  • Species
    Synthetic
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc-mRuby2 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer AGG TCT ATA TAA GCA GAG CTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLEX.(Cyto)iGlucoSnFR.H66A.L276V-mRuby2 was a gift from Paul Khavari (Addgene plasmid # 238943 ; http://n2t.net/addgene:238943 ; RRID:Addgene_238943)

  • For your References section:

    Glucose modulates IRF6 transcription factor dimerization to enable epidermal differentiation. Lopez-Pajares V, Bhaduri A, Zhao Y, Gowrishankar G, Donohue LKH, Guo MG, Siprashvili Z, Miao W, Nguyen DT, Yang X, Li AM, Tung AS, Shanderson RL, Winge MCG, Meservey LM, Srinivasan S, Meyers RM, Guerrero A, Ji AL, Garcia OS, Tao S, Gambhir SS, Long JZ, Ye J, Khavari PA. Cell Stem Cell. 2025 Mar 20:S1934-5909(25)00088-8. doi: 10.1016/j.stem.2025.02.017. 10.1016/j.stem.2025.02.017 PubMed 40120584
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