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pAAV_hSyn1_SypHer3s_mito
(Plasmid #238972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238972 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAAV9
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SypHer3s
  • Species
    Synthetic
  • Promoter hSyn1
  • Tag / Fusion Protein
    • 3x Cytochrome C Oxidase Subunit VIII presequense (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also named pAAV9_hSyn1_SypHer3s_mito in Kelmanson et al. 2021.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV_hSyn1_SypHer3s_mito was a gift from Dmitry Bilan (Addgene plasmid # 238972 ; http://n2t.net/addgene:238972 ; RRID:Addgene_238972)

  • For your References section:

    In vivo dynamics of acidosis and oxidative stress in the acute phase of an ischemic stroke in a rodent model. Kelmanson IV, Shokhina AG, Kotova DA, Pochechuev MS, Ivanova AD, Kostyuk AI, Panova AS, Borodinova AA, Solotenkov MA, Stepanov EA, Raevskii RI, Moshchenko AA, Pak VV, Ermakova YG, van Belle GJC, Tarabykin V, Balaban PM, Fedotov IV, Fedotov AB, Conrad M, Bogeski I, Katschinski DM, Doeppner TR, Bahr M, Zheltikov AM, Belousov VV, Bilan DS. Redox Biol. 2021 Nov 3;48:102178. doi: 10.1016/j.redox.2021.102178. 10.1016/j.redox.2021.102178 PubMed 34773835
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