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Addgene

pVeBtA1
(Plasmid #238986)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238986 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    arrestin-1
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    2000
  • Mutation
    none
  • Entrez Gene
    SAG (a.k.a. S-AG)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco RI (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVeBtA1 was a gift from Vsevolod Gurevich (Addgene plasmid # 238986 ; http://n2t.net/addgene:238986 ; RRID:Addgene_238986)

  • For your References section:

    Role of receptor-attached phosphates in binding of visual and non-visual arrestins to G protein-coupled receptors. Gimenez LE, Kook S, Vishnivetskiy SA, Ahmed MR, Gurevich EV, Gurevich VV. J Biol Chem. 2012 Mar 16;287(12):9028-40. doi: 10.1074/jbc.M111.311803. Epub 2012 Jan 24. 10.1074/jbc.M111.311803 PubMed 22275358
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