pGP-JFRC59-13XLexAop2-IVS-Syn21-Positron2-p10
(Plasmid
#239081)
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PurposeLexA activated transgene expression of positive-going voltage sensor under the hsp70 promoter in D. melanogaster
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239081 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJFRC59
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Backbone manufacturerGerald Rubin
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namePositron2
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Alt namePositron2 insert
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Alt namevariant 177.6237
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SpeciesSynthetic
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Insert Size (bp)1659
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MutationR78K N81D D92N W178F
- Promoter hsp70
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGP-JFRC59-13XLexAop2-IVS-Syn21-Positron2-p10 was a gift from GENIE Project (Addgene plasmid # 239081 ; http://n2t.net/addgene:239081 ; RRID:Addgene_239081) -
For your References section:
Optimization of Ace2N based voltage indicators. Eric R. Schreiter, Ahmed Abdelfattah, Jeremy Hasseman, Daniel Reep, Getahun Tsegaye, Arthur Tsang, Ilya Kolb, Allan Wong, Amy Chuong, Jihong Zheng, Ben Arthur, Wyatt Korff, GENIE Project Team. Janelia Figshare 2023 10.25378/janelia.22325446.v1