pmScarlet3-H_I3
(Plasmid
#239343)
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PurposeExpresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5321
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameI3-01
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Alt nameI3 nanocage subunit
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SpeciesSynthetic
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Insert Size (bp)609
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MutationK129A
- Promoter CMV
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Tag
/ Fusion Protein
- mScarlet3-H (a.k.a. mYongHong) (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmScarlet3-H_I3 was a gift from Torsten Wittmann (Addgene plasmid # 239343 ; http://n2t.net/addgene:239343 ; RRID:Addgene_239343)