pmScarlet3-H_I3
(Plasmid #239343)

• Purpose: Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.
• Depositing Lab: Torsten Wittmann
• Publication: Quantitative Comparison of Fluorescent Proteins using Protein Nanocages in Live Cells (unpublished)

Backbone

• Vector backbone: pEGFP
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5321
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: I3-01
• Alt name: I3 nanocage subunit
• Species: Synthetic
• Insert Size (bp): 609
• Mutation: K129A
• Promoter: CMV
• Tag / Fusion Protein:
  • mScarlet3-H (a.k.a. mYongHong) (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • mScarlet-3H_I3.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry