pcDNA5/FRT/MALAT1[WT] (pAVA3171)
(Plasmid
#239347)
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PurposeExpresses full-length, driven by its own promoter MALAT1 with wild-type ENE and an 11-nt insertion (cgctcgacgta).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 239347 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepcDNA5/FRT
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Vector typeMammalian Expression
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Selectable markersATG-lacking-HygroR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name10,843 bp of MALAT1 of genomic locus amplified from genomic DNA of Hela cells
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SpeciesH. sapiens (human)
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MutationAn 11-nt insertion (cgctcgacgta)
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Entrez GeneMALAT1 (a.k.a. HCN, LINC00047, NCRNA00047, NEAT2, PRO2853)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.26.615073 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5/FRT/MALAT1[WT] (pAVA3171) was a gift from Andrei Alexandrov (Addgene plasmid # 239347 ; http://n2t.net/addgene:239347 ; RRID:Addgene_239347) -
For your References section:
Identification of human pathways acting on nuclear non-coding RNAs using the Mirror forward genetic approach. Che R, Panah M, Mirani B, Knowles K, Ostapovich A, Majumdar D, Chen X, DeSimone J, White W, Noonan M, Luo H, Alexandrov A. Nat Commun. 2025 May 22;16(1):4741. doi: 10.1038/s41467-025-59998-3. 10.1038/s41467-025-59998-3 PubMed 40399278