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Addgene

p4919
(Plasmid #239357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-T (?)
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2973
  • Total vector size (bp) 9207
  • Modifications to backbone
    Addition of PacI site
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rice CUL1, Rice SKP1, Rice RBX1 and G418 resistance
  • Alt name
    CUL1 = CDC53 = Cullin
  • Species
    Oryza sativa
  • Insert Size (bp)
    6237
  • GenBank ID
    CUL1: XP_015619069 SKP1: NP_001411237. RBX1: XP_015625781.1 G418 resistance: AAO21931.1
  • Promoter none
  • Tag / Fusion Protein
    • CUL1, SKP1 and RBX1 are all Ty epitope tagged (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4919 was a gift from Mark Carrington (Addgene plasmid # 239357 ; http://n2t.net/addgene:239357 ; RRID:Addgene_239357)

  • For your References section:

    An auxin-inducible degron system for trypanosomes. Alexandra Klein, Alice McDowell, Christian R S Reis, Rodrigo Pontes de Lima, Danielle M N Moura, Janaina de Freitas Nascimento, Osvaldo P de Melo Neto, Mark Carrington. bioRxiv 2025.06.17.660065 10.1101/2025.06.17.660065
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