p5179
(Plasmid #239358)

• Purpose: Exrpession of Rice TIR1 F74->G, Rice ARF fragment and blaticidin resistance by read through transaction after integration int the PFR1 locus
• Depositing Lab: Mark Carrington
• Publication: Alexandra et al bioRxiv 2025.06.17.660065

Backbone

• Vector backbone: pGEM-T
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2936
• Total vector size (bp): 7424
• Modifications to backbone: insertion PacI site
• Vector type: bacterial

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rice TIR1, Rice ARF16 and blasticidin resistance
• Species: Oryza sativa
• Insert Size (bp): 4536
• Mutation: TIR1 F74->G. ARF16 is residues 784 to 959 only
• GenBank ID: ARF16: XP_015643022 TIR1: NP_001389282.1
• Promoter: none
• Tag / Fusion Protein:
  • TIR1 and ARF are both Ty-epitope tagged (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry