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pPOTv6-hyg-3Ty:OsAID:3Ty (p5230)
(Plasmid #239362)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239362 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPOTv7-hyg
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 6280
  • Total vector size (bp) 6991
  • Vector type
    bacterial
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rice Auxin inducible degradation domain
  • Alt name
    AID
  • Species
    Rice
  • Insert Size (bp)
    711
  • Promoter n/a
  • Tags / Fusion Proteins
    • 3 x Ty epitope tag (N terminal on backbone)
    • 3 x Ty epitope tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer GCGATAGCTTAGCTATCAG
  • 3′ sequencing primer AGTGCACGTACACAGCGGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPOTv6-hyg-3Ty:OsAID:3Ty (p5230) was a gift from Mark Carrington (Addgene plasmid # 239362 ; http://n2t.net/addgene:239362 ; RRID:Addgene_239362)

  • For your References section:

    An auxin-inducible degron system for trypanosomes. Alexandra Klein, Alice McDowell, Christian R S Reis, Rodrigo Pontes de Lima, Danielle M N Moura, Janaina de Freitas Nascimento, Osvaldo P de Melo Neto, Mark Carrington. bioRxiv 2025.06.17.660065 10.1101/2025.06.17.660065
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