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Addgene

pAAV2ST
(Plasmid #239400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC57-KAN
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 4314
  • Total vector size (bp) 5871
  • Modifications to backbone
    Added a TetR spacer element to separate the origin of replication from the proximal ITR.
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Green fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    1557
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer CMV_F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV2ST was a gift from Fred Bunz (Addgene plasmid # 239400 ; http://n2t.net/addgene:239400 ; RRID:Addgene_239400)
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