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Addgene

pTom20-mScarlet3-H (aka mYongHong)
(Plasmid #239412)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239412 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pN1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tom20
  • Species
    H. sapiens (human)
  • Mutation
    M163H in mScarlet3
  • Entrez Gene
    TOMM20 (a.k.a. MAS20, MOM19, TOM20)
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet3-H (aka mYongHong) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTom20-mScarlet3-H (aka mYongHong) was a gift from Zhifei Fu (Addgene plasmid # 239412 ; http://n2t.net/addgene:239412 ; RRID:Addgene_239412)
  • For your References section:

    A highly stable monomeric red fluorescent protein for advanced microscopy. Xiong H, Chang Q, Ding J, Wang S, Zhang W, Li Y, Wu Y, Lin P, Yang C, Liu M, Fang G, Yang Y, Xie J, Qi D, Jiang T, Fu W, Hu F, Chen Y, Yue R, Li Y, Cui Y, Li M, Fan S, Yang Y, Xu Y, Li D, Zhang F, Zhao H, Wu C, Zheng Q, Piatkevich KD, Fu Z. Nat Methods. 2025 Apr 17. doi: 10.1038/s41592-025-02676-5. 10.1038/s41592-025-02676-5 PubMed 40247125