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Addgene

pACYC-prk:nptII
(Plasmid #239580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239580 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACYC
  • Backbone size w/o insert (bp) 5094
  • Total vector size (bp) 6882
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRK-NPTII
  • Species
    Synthetic
  • Insert Size (bp)
    1788
  • GenBank ID
    ATN39931.1

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Prof. Spencer Whitney.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PRK-NPTII insert is prone to excision via recombination — consider recombination deficient strains or lower growth temperature if this occurs routinely.

Please visit https://doi.org/10.1101/2025.02.17.638297 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYC-prk:nptII was a gift from Matthew D. Shoulders (Addgene plasmid # 239580 ; http://n2t.net/addgene:239580 ; RRID:Addgene_239580)
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