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BAC-GWS1B
(Plasmid #239581)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239581 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    BAC
  • Backbone manufacturer
    Addgene (85710)
  • Backbone size w/o insert (bp) 8029
  • Total vector size (bp) 9420
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GWS1B Rubisco (6X his)
  • Species
    Gallionellaceae
  • Insert Size (bp)
    1392
  • Mutation
    Wild-type
  • GenBank ID
    ALP32085.1
  • Promoter T7, tac
  • Tag / Fusion Protein
    • 6X histag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aacaggaaggcaaaatgccg
  • 3′ sequencing primer ataaagtgcaagcaatacgc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.17.638297 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BAC-GWS1B was a gift from Matthew D. Shoulders (Addgene plasmid # 239581 ; http://n2t.net/addgene:239581 ; RRID:Addgene_239581)

  • For your References section:

    In vivo directed evolution of an ultrafast Rubisco from a semianaerobic environment imparts oxygen resistance. McDonald JL, Shapiro NP, Mengiste AA, Kaines S, Whitney SM, Wilson RH, Shoulders MD. Proc Natl Acad Sci U S A. 2025 Jul 8;122(27):e2505083122. doi: 10.1073/pnas.2505083122. Epub 2025 Jun 30. 10.1073/pnas.2505083122 PubMed 40587785
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