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Addgene

pTrc-GWS1B
(Plasmid #239582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239582 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTrc
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4307
  • Total vector size (bp) 5704
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GWS1B Rubisco (6X his)
  • Species
    Gallionellaceae
  • Insert Size (bp)
    1395
  • Mutation
    Wild-type
  • GenBank ID
    ALP32085.1
  • Promoter Trc
  • Tag / Fusion Protein
    • 6X histag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcgcaacgcaattaatgtaa
  • 3′ sequencing primer aaattctgttttatcagacc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.02.17.638297 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTrc-GWS1B was a gift from Matthew D. Shoulders (Addgene plasmid # 239582 ; http://n2t.net/addgene:239582 ; RRID:Addgene_239582)
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