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nCaMK2rep-S603A
(Plasmid #239625)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239625 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLL3.7
  • Backbone size w/o insert (bp) 6324
  • Total vector size (bp) 7098
  • Modifications to backbone
    Vector backbone is derived from the plasmid SYN-DsRed-SYN-GFP (S.Gascon et al., 2008). The plasmid was digested using the enzymes BamHI and NheI, resulting in a single promoter
  • Vector type
    Mammalian Expression, Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PSD95.FingR-NES-SynP3-3xmyc
  • Species
    R. norvegicus (rat), Synthetic
  • Insert Size (bp)
    774
  • Mutation
    Changed serine 603 to Alanine
  • GenBank ID
    NM_019133.2
  • Entrez Gene
    Syn1
  • Promoter hSyn
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CTCAGTCTGCGGTGGGCAGC
  • 3′ sequencing primer CAACATAGTTAAGAATACCAGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.05.07.652623 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nCaMK2rep-S603A was a gift from F Javier Díez Guerra (Addgene plasmid # 239625 ; http://n2t.net/addgene:239625 ; RRID:Addgene_239625)
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