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pNluc-hbb
(Plasmid #239753)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239753 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGL3Control
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3636
  • Total vector size (bp) 4336
  • Modifications to backbone
    Introduction of multiple cloning site at 3' end of luciferase allowing restriction cloning to replace gene.
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nanoluciferase flanked by human haemoglobin beta 5' and 3' UTRs
  • Alt name
    Nluc
  • Species
    Engineered reporter (from Promega)
  • Insert Size (bp)
    700
  • GenBank ID
    JQ437370.1
  • Promoter T7 (included as part of insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TCTGCGATCTGCATCTCAATTA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Designed as a Gene Block using Promega Nluc sequence.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNluc-hbb was a gift from Catherine Jopling (Addgene plasmid # 239753 ; http://n2t.net/addgene:239753 ; RRID:Addgene_239753)

  • For your References section:

    A proximity-labeling-based approach to directly detect mRNA delivery to specific subcellular locations. Smart AD, Hughes ME, Ruiz Velasco AD, Hori N, Stolnik S, Jopling CL. Mol Ther Nucleic Acids. 2025 Jun 24;36(3):102602. doi: 10.1016/j.omtn.2025.102602. eCollection 2025 Sep 9. 10.1016/j.omtn.2025.102602 PubMed 40661784
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