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Addgene

hNSE-NF186(K519TAG)-HA
(Plasmid #239782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    modified pEGFP-N1 (pEGFP was removed from the pEGFP-N1 backbone)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nfasc
  • Species
    R. norvegicus (rat)
  • Mutation
    K519TAG
  • Entrez Gene
    Nfasc (a.k.a. NF)
  • Promoter hNSE (obtained from Addgene plasmid #11606)
  • Tag / Fusion Protein
    • HA-tag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Vann Bennett (NF186 gene was derived from Addgene plasmid #31061), and Rosalyn Adam (hNSE promoter was derived from Addgene plasmid #11606).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Neurofascin-186-HA plasmid was obtained from Addgene (plasmid #31061). The HA tag was moved from N to C terminus, and CMV promoter was replaced with hNSE (obtained from Addgene plasmid #11606)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hNSE-NF186(K519TAG)-HA was a gift from Ivana Nikić-Spiegel (Addgene plasmid # 239782 ; http://n2t.net/addgene:239782 ; RRID:Addgene_239782)

  • For your References section:

    Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry. Stajkovic N, Liu Y, Arsic A, Meng N, Lyu H, Zhang N, Grimm D, Lerche H, Nikic-Spiegel I. J Cell Sci. 2023 Jun 15;136(12):jcs260600. doi: 10.1242/jcs.260600. Epub 2023 Jun 28. 10.1242/jcs.260600 PubMed 37288813
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