ATP5F1B-turboGFP
(Plasmid
#239795)
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PurposeExpresses human ATP5F1B tagged with turboGFP
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Depositing Lab
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Depositing OrganizationUniversity of California, Berkeley
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239795 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 239795-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 239795-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV6-AC-GFP
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Backbone manufacturerOrigene
- Backbone size w/o insert (bp) 6600
- Total vector size (bp) 8096
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameATP5F1b
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Alt nameATP5B
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SpeciesH. sapiens (human)
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Insert Size (bp)1583
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GenBank IDNM_001686
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Entrez GeneATP5F1B (a.k.a. ATP5B, ATPMB, ATPSB, HEL-S-271, HUMOP2)
- Promoter CMV
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Tag
/ Fusion Protein
- turboGFP (C terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer CMV Forward
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 239795-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 239795-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ATP5F1B-turboGFP was a gift from Samantha Lewis (Addgene plasmid # 239795 ; http://n2t.net/addgene:239795 ; RRID:Addgene_239795) -
For your References section:
In situ cryo-ET visualization of mitochondrial depolarization and mitophagic engulfment. Rose K, Herrmann E, Kakudji E, Lizarrondo J, Celebi AY, Wilfling F, Lewis SC, Hurley JH. Proc Natl Acad Sci U S A. 2025 Aug 5;122(31):e2511890122. doi: 10.1073/pnas.2511890122. Epub 2025 Jul 31. 10.1073/pnas.2511890122 PubMed 40743392
