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Addgene

PiggyBac-FLAG-hTET2-IRES-Blast
(Plasmid #239819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239819 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    PiggyBac
  • Total vector size (bp) 13232
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG-tagged human TET2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6069
  • GenBank ID
    NM_001127208 54790
  • Tag / Fusion Protein
    • 2x FLAG tag on TET2 (N terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PiggyBac-FLAG-hTET2-IRES-Blast was a gift from Kasper Rasmussen (Addgene plasmid # 239819 ; http://n2t.net/addgene:239819 ; RRID:Addgene_239819)

  • For your References section:

    The TET protein family interactor PROSER1 sustains hematopoietic stem cell function. Knatko EV, Fleming A, Li X, Crawley P, Budriunaite I, Rasmussen KD. Blood Adv. 2025 Jun 24:bloodadvances.2024015683. doi: 10.1182/bloodadvances.2024015683. 10.1182/bloodadvances.2024015683 PubMed 40554416
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