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pACEBac1-eIF3a-TEV-FLAG
(Plasmid #240024)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240024 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    Geneva Biotech
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF3a
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    EIF3A (a.k.a. EIF3, EIF3S10, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • TEV_3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACEBac1-eIF3a-TEV-FLAG was a gift from Venki Ramakrishnan (Addgene plasmid # 240024 ; http://n2t.net/addgene:240024 ; RRID:Addgene_240024)

  • For your References section:

    Purification and characterization of recombinant human translation initiation factor eIF3. Diaz-Lopez I, Gordiyenko Y, Zuber PK, Li X, Ramakrishnan V. Protein Sci. 2026 Jan;35(1):e70388. doi: 10.1002/pro.70388. 10.1002/pro.70388 PubMed 41432235
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