pAAV-CaMKII-Ace-mNeon1-WPRE
(Plasmid
#240053)
-
PurposeConstitutive expression of genetically encoded voltage indicator Ace-mNeon1
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 240053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneAAV
-
Vector typeAAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAce-mNeon1
-
Alt nameAce2N-4AA-mNeon
-
SpeciesSynthetic
- Promoter CaMKII
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byGong Y, Huang C, Li JZ, Grewe BF, Zhang Y, Eismann S, Schnitzer MJ. (2015) High-speed recording of neural spikes in awake mice and flies with a fluorescent voltage sensor. Science. 350(6266):1361-1366.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-CaMKII-Ace-mNeon1-WPRE was a gift from Mark Schnitzer (Addgene plasmid # 240053 ; http://n2t.net/addgene:240053 ; RRID:Addgene_240053) -
For your References section:
Imaging high-frequency voltage dynamics in multiple neuron classes of behaving mammals. Haziza S, Chrapkiewicz R, Zhang Y, Kruzhilin V, Li J, Li J, Delamare G, Swanson R, Buzsaki G, Kannan M, Vasan G, Lin MZ, Zeng H, Daigle TL, Schnitzer MJ. Cell, 188, 1-23, August 7, 2025. doi: 10.1016/j.cell.2025.06.028 10.1016/j.cell.2025.06.028 PubMed 39185175