peGFP-HDAC1 (pc2447)
(Plasmid
#240148)
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PurposeExpression vector containing a fusion protein of eGFP and human HDAC1.
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Depositing Lab
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Depositing OrganizationTechnische Universitaet Darmstadt
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 240148-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 240148-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 6204
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHDAC1
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SpeciesH. sapiens (human)
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Insert Size (bp)1442
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Entrez GeneHDAC1 (a.k.a. GON-10, HD1, KDAC1, RPD3, RPD3L1)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer AGAAGCGCGATCACATG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS finds L41M in HDAC1. The depositor confirms that discrepancies between the depositor's sequence and Addgene's sequences have no functional consequences.
DNA (Catalog # 240148-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 240148-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
peGFP-HDAC1 (pc2447) was a gift from Cristina Cardoso (Addgene plasmid # 240148 ; http://n2t.net/addgene:240148 ; RRID:Addgene_240148) -
For your References section:
Developmental differences in genome replication program and origin activation. Rausch C, Weber P, Prorok P, Horl D, Maiser A, Lehmkuhl A, Chagin VO, Casas-Delucchi CS, Leonhardt H, Cardoso MC. Nucleic Acids Res. 2020 Dec 16;48(22):12751-12777. doi: 10.1093/nar/gkaa1124. 10.1093/nar/gkaa1124 PubMed 33264404
Map uploaded by the depositor.
