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pET15b-ALFA-TwinStrep
(Plasmid #240252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240252 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-15b
  • Modifications to backbone
    Altered multiple cloning site, codon-optimized thrombin cleavage site, codon-optimized tags (ALFA, Twin-Strep, 10x HIS)
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • Thrombin (C terminal on backbone)
    • ALFA (C terminal on backbone)
    • Twin-Strep (C terminal on backbone)
    • 10x HIS (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The BL21(DE3) strain and derivatives should be used for expression.
  • Copy number
    High Copy

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For compatible plasmids, please see: Addgene plasmid #159986 and #159987.

Please visit https://doi.org/10.1101/2023.12.03.569786 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-ALFA-TwinStrep was a gift from Olga Boudker & Krishna Reddy (Addgene plasmid # 240252 ; http://n2t.net/addgene:240252 ; RRID:Addgene_240252)

  • For your References section:

    Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters. Reddy KD, Rasool B, Akher FB, Kutlesic N, Pant S, Boudker O. Nat Struct Mol Biol (2025). 10.1038/s41594-025-01652-z PubMed 38106174
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