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Addgene

Vim-mCh-SspB
(Plasmid #240421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240421 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pB80
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vimentin
  • Species
    H. sapiens (human)
  • Entrez Gene
    VIM
  • Tags / Fusion Proteins
    • mCherry (C terminal on insert)
    • SspB (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.21.624725 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Vim-mCh-SspB was a gift from Anna Akhmanova (Addgene plasmid # 240421 ; http://n2t.net/addgene:240421 ; RRID:Addgene_240421)

  • For your References section:

    Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments. Pasolli M, Meiring JCM, Conboy JP, Koenderink GH, Akhmanova A. J Cell Biol. 2025 Sep 1;224(9):e202504004. doi: 10.1083/jcb.202504004. Epub 2025 Jul 8. 10.1083/jcb.202504004 PubMed 40627079
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