Halo-mitoTag
(Plasmid
#240424)
-
PurposeVisualization of mitochondria
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 240424 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepGFP-N1
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameactA
-
Insert Size (bp)78
-
Tag
/ Fusion Protein
- Halotag (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.21.624725 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
Halo-mitoTag was a gift from Anna Akhmanova (Addgene plasmid # 240424 ; http://n2t.net/addgene:240424 ; RRID:Addgene_240424) -
For your References section:
Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments. Pasolli M, Meiring JCM, Conboy JP, Koenderink GH, Akhmanova A. J Cell Biol. 2025 Sep 1;224(9):e202504004. doi: 10.1083/jcb.202504004. Epub 2025 Jul 8. 10.1083/jcb.202504004 PubMed 40627079