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HisH2A-InteinCBD-H2B
(Plasmid #240442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240442 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDUET1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    HisH2A-Intein-CBD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1255
  • Tags / Fusion Proteins
    • His tag (N terminal on insert)
    • C-terminal fusion with intein-chitin binding domain (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    H2B
  • Insert Size (bp)
    381
  • Entrez Gene
    H2B

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HisH2A-InteinCBD-H2B was a gift from Mark Glover (Addgene plasmid # 240442 ; http://n2t.net/addgene:240442 ; RRID:Addgene_240442)

  • For your References section:

    Structural insights into gammaH2Ax containing nucleosomes. Panigrahi R, Edwards R, Islam MT, Lu J, Kulepa A, Kim TH, Mark Glover JN. Nucleic Acids Res. 2025 Oct 14;53(19):gkaf1028. doi: 10.1093/nar/gkaf1028. 10.1093/nar/gkaf1028 PubMed 41123210
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