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pBI121::chitGFP5:AQ
(Plasmid #240450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240450 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBI121
  • Backbone size w/o insert (bp) 12868
  • Vector type
    Bacterial Expression, Plant Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli: 37°C A. tumefaciens C58: 28°C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Fusion of Chitinase signal, smGFP5, and Aequorin
  • Species
    Synthetic
  • Insert Size (bp)
    1633
  • Mutation
    GFP5 for expression plants (PMID 9122158); Soluble modified (PMID 9484447)
  • Promoter Cauliflower mosaic virus 35S promoter (GenBank AJ007625) without artificial 40 bp insert drives expression plants

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site SacI (unknown if destroyed)
  • 5′ sequencing primer 5' GTTCATTTCATTTGGAGAG 3'
  • 3′ sequencing primer 5' ATTCGAGCTCGGTACC 3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See the Supplemental Document under Resource Information for more details, including annotated maps. Figure 1 of the Gao et al. (2004) paper also gives an overview of the gene cassettes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI121::chitGFP5:AQ was a gift from Christoph Plieth (Addgene plasmid # 240450 ; http://n2t.net/addgene:240450 ; RRID:Addgene_240450)

  • For your References section:

    Self-reporting Arabidopsis expressing pH and [Ca2+] indicators unveil ion dynamics in the cytoplasm and in the apoplast under abiotic stress. Gao D, Knight MR, Trewavas AJ, Sattelmacher B, Plieth C. Plant Physiol. 2004 Mar;134(3):898-908. doi: 10.1104/pp.103.032508. 10.1104/pp.103.032508 PubMed 15020753
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