pBI121::chitGFP5:AQ
(Plasmid
#240450)
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PurposeExpression of indicator protein fusion (soluble modified GFP5 & Aequorin) for monitoring calcium concentrations in cell walls of higher plants. For details, see Comments.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240450 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepBI121
- Backbone size w/o insert (bp) 12868
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Vector typeBacterial Expression, Plant Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsE. coli: 37°C A. tumefaciens C58: 28°C
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameFusion of Chitinase signal, smGFP5, and Aequorin
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SpeciesSynthetic
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Insert Size (bp)1633
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MutationGFP5 for expression plants (PMID 9122158); Soluble modified (PMID 9484447)
- Promoter Cauliflower mosaic virus 35S promoter (GenBank AJ007625) without artificial 40 bp insert drives expression plants
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site SacI (unknown if destroyed)
- 5′ sequencing primer 5' GTTCATTTCATTTGGAGAG 3'
- 3′ sequencing primer 5' ATTCGAGCTCGGTACC 3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBI121::chitGFP5:AQ was a gift from Christoph Plieth (Addgene plasmid # 240450 ; http://n2t.net/addgene:240450 ; RRID:Addgene_240450) -
For your References section:
Self-reporting Arabidopsis expressing pH and [Ca2+] indicators unveil ion dynamics in the cytoplasm and in the apoplast under abiotic stress. Gao D, Knight MR, Trewavas AJ, Sattelmacher B, Plieth C. Plant Physiol. 2004 Mar;134(3):898-908. doi: 10.1104/pp.103.032508. 10.1104/pp.103.032508 PubMed 15020753