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pBI121::chitRatioGFP:LAAQ
(Plasmid #240451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240451 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBI121
  • Backbone size w/o insert (bp) 12868
  • Vector type
    Bacterial Expression, Plant Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli: 37°C A. tumefaciens C58: 28°C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Fusion of chitinase signal, soluble modified ratiometric pHluorin and low affinity Aequorin (D119A)
  • Species
    Synthetic
  • Insert Size (bp)
    1633
  • Mutation
    Ratiometric GFP (ratiometric pHluorin) (PMID 9671304); Soluble modified for expression in plants (PMIDs 9122158,9484447); Low affinity Aequorin (D119A mutant) (PMID 1530606)
  • Promoter Cauliflower mosaic virus 35S promoter (GenBank AJ007625) without artificial 40 bp insert drives expression plants

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site SacI (unknown if destroyed)
  • 5′ sequencing primer 5' GTTCATTTCATTTGGAGAG 3'
  • 3′ sequencing primer 5' ATTCGAGCTCGGTACC 3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBI121::chitRatioGFP:LAAQ was a gift from Christoph Plieth (Addgene plasmid # 240451 ; http://n2t.net/addgene:240451 ; RRID:Addgene_240451)

  • For your References section:

    Self-reporting Arabidopsis expressing pH and [Ca2+] indicators unveil ion dynamics in the cytoplasm and in the apoplast under abiotic stress. Gao D, Knight MR, Trewavas AJ, Sattelmacher B, Plieth C. Plant Physiol. 2004 Mar;134(3):898-908. doi: 10.1104/pp.103.032508. 10.1104/pp.103.032508 PubMed 15020753
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