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CARMEN
(Plasmid #240464)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240464 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Gene Universal
  • Backbone size w/o insert (bp) 5332
  • Total vector size (bp) 10903
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CARMEN
  • Alt name
    CEPIA3mt + T2A + mTagBFP2-NLS + P2A + NIR-GECO2G-NES + E2A + R-CEPIA1er
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5571

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CARMEN was a gift from Roland Malli (Addgene plasmid # 240464 ; http://n2t.net/addgene:240464 ; RRID:Addgene_240464)

  • For your References section:

    A Quad-Cistronic Fluorescent Biosensor System for Real-Time Detection of Subcellular Ca²⁺ Signals. Lischnig A, Erdoğan YC, Gottschalk B, Gruber M, Groselj-Strele A, Burgstaller S, Graier WR, Malli R. British Journal of Pharmacology 2025 10.1111/bph.70211
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