pRK172 asyn(1-103)
(Plasmid
#240597)
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PurposeOverexpress truncated alpha-Synuclein protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 240597 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepRK172
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameaSyn
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SpeciesH. sapiens (human)
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Mutationaa 1-103 only
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Entrez GeneSNCA (a.k.a. NACP, PARK1, PARK4, PD1)
- Promoter T7
Cloning Information
- Cloning method Other
- 5′ sequencing primer T7 fwd (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK172 asyn(1-103) was a gift from Jennifer Lee (Addgene plasmid # 240597 ; http://n2t.net/addgene:240597 ; RRID:Addgene_240597) -
For your References section:
Structural Insights into alpha-Synuclein Fibril Polymorphism: Effects of Parkinson's Disease-Related C-Terminal Truncations. Ni X, McGlinchey RP, Jiang J, Lee JC. J Mol Biol. 2019 Sep 6;431(19):3913-3919. doi: 10.1016/j.jmb.2019.07.001. Epub 2019 Jul 8. 10.1016/j.jmb.2019.07.001 PubMed 31295458