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Addgene

p2T-TetON-Hygro-Arhgef11-Cry2-mCh
(Plasmid #240798)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240798 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pminiTol2
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Arhgef11-Cry2-mCherry
  • Species
    Synthetic
  • Mutation
    none
  • Promoter TRE

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2T-TetON-Hygro-Arhgef11-Cry2-mCh was a gift from Hans Clevers (Addgene plasmid # 240798 ; http://n2t.net/addgene:240798 ; RRID:Addgene_240798)
  • For your References section:

    Epithelial tension controls intestinal cell extrusion. Krueger D, Spoelstra WK, Mastebroek DJ, Kok RNU, Wu S, Nikolaev M, Bannier-Helaouet M, Gjorevski N, Lutolf M, van Es J, van Zon J, Tans SJ, Clevers H. Science. 2025 Sep 4;389(6764):eadr8753. doi: 10.1126/science.adr8753. Epub 2025 Sep 4. 10.1126/science.adr8753 PubMed 40906841