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pSS2.1-Cas9-HH-gRNADhps-HDV
(Plasmid #241015)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241015 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pSS2.1
  • Backbone manufacturer
    Steven Sayson, Ph.D.
  • Backbone size w/o insert (bp) 5320
  • Total vector size (bp) 9641
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Cas9-Hammerhead (HH) Ribozyme-gRNA(Dhps)-Hepatitis Delta Virus (HDV) Ribozyme
  • Alt name
    Cas9-HH-gRNA(Dhps)-HDV
  • Insert Size (bp)
    4321

Gene/Insert 2

  • Gene/Insert name
    blasticidin-S deaminase
  • Alt name
    Bsd
  • Alt name
    Bsr
  • Insert Size (bp)
    423

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSS2.1-Cas9-HH-gRNADhps-HDV was a gift from Alan Smulian (Addgene plasmid # 241015 ; http://n2t.net/addgene:241015 ; RRID:Addgene_241015)

  • For your References section:

    Extracellular vesicle-mediated delivery of genetic material for transformation and CRISPR/Cas9-based gene editing in Pneumocystis murina. Sayson SG, Ashbaugh A, Bauer LC, Smulian AG. mBio. 2025 Sep 23:e0182525. doi: 10.1128/mbio.01825-25. 10.1128/mbio.01825-25 PubMed 40985725
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