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pDMJ2-SARS-CoV-2-Spike (BA.5-2f)
(Plasmid #241103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241103 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDMJ2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spike (BA.5-2f)
  • Species
    SARS-CoV-2
  • Mutation
    T76I,M153S,T19I,L24del,P25del,P26del,A27S,H69del,V70del,G142D,V213G,G339D,S371F,S373P,S375F,T376A,D405N,R408S,K417N,N440K,L452R,S477N,T478K,E484A,F486V,Q498R,N501Y,Y505H,D614G,H655Y,N679K,P681H,N764K,D796Y,Q954H,N969K
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)

Cloning Information

  • Cloning method Gene Synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDMJ2-SARS-CoV-2-Spike (BA.5-2f) was a gift from Jeremy Luban (Addgene plasmid # 241103 ; http://n2t.net/addgene:241103 ; RRID:Addgene_241103)

  • For your References section:

    Computationally designed proteins mimic antibody immune evasion in viral evolution. Youssef N, Gurev S, Ghantous F, Brock KP, Jaimes JA, Thadani NN, Dauphin A, Sherman AC, Yurkovetskiy L, Soto D, Estanboulieh R, Kotzen B, Notin P, Kollasch AW, Cohen AA, Dross SE, Erasmus J, Fuller DH, Bjorkman PJ, Lemieux JE, Luban J, Seaman MS, Marks DS. Immunity. 2025 May 6:S1074-7613(25)00178-5. doi: 10.1016/j.immuni.2025.04.015. 10.1016/j.immuni.2025.04.015 PubMed 40345199
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