GfaABC1D-Lck-mCherry-ibARK
(Plasmid
#241241)
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PurposeExpression of plasma membrane-tethered ibARK in astrocytes
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241241 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneUnknown
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLck-mCherry-ibARK
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SpeciesB. taurus (bovine)
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: This plasmid contains a 22-bp deletion within the 5' ITR. Despite this deletion, the vector consistently packaged, expressed, and produced the reported phenotypes in the depositor's experiments.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GfaABC1D-Lck-mCherry-ibARK was a gift from Jun Nagai (Addgene plasmid # 241241 ; http://n2t.net/addgene:241241 ; RRID:Addgene_241241) -
For your References section:
The astrocytic ensemble acts as a multiday trace to stabilize memory. Dewa K, Kaseda K, Kuwahara A, Kubotera H, Yamasaki A, Awata N, Komori A, Holtz MA, Kasai A, Skibbe H, Takata N, Yokoyama T, Tsuda M, Numata G, Nakamura S, Takimoto E, Sakamoto M, Ito M, Masuda T, Nagai J. Nature (2025) 10.1038/s41586-025-09619-2