pSS02 NAPstarC
(Plasmid
#241271)
-
PurposeExpresses NADPH/NADP+ biosensor NAPstarC in Arabidopsis thaliana
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 241271 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepSS02
- Backbone size w/o insert (bp) 9954
- Total vector size (bp) 12723
-
Vector typePlant Expression
-
Selectable markersHygromycin
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namecyt-NAPstarC
-
SpeciesAequorea victoria; Thermus aquaticus, Discosoma sp.
-
Insert Size (bp)2769
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made bythe original Peredox-mCherry sequence came from Gary Yellen’s lab via Addgene
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSS02 NAPstarC was a gift from Markus Schwarzländer (Addgene plasmid # 241271 ; http://n2t.net/addgene:241271 ; RRID:Addgene_241271) -
For your References section:
A family of NADPH/NADP(+) biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes. Scherschel M, Niemeier JO, Jacobs LJHC, Hoffmann MDA, Diederich A, Bell C, Hohne P, Raetz S, Kroll JB, Steinbeck J, Lichtenauer S, Multhoff J, Zimmermann J, Sadhanasatish T, Rothemann RA, Grashoff C, Messens J, Ampofo E, Laschke MW, Riemer J, Roma LP, Schwarzlander M, Morgan B. Nat Commun. 2024 Dec 19;15(1):10704. doi: 10.1038/s41467-024-55302-x. 10.1038/s41467-024-55302-x PubMed 39702652