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pANAP-P2A-eRF(E55D)
(Plasmid #241290)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241290 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pANAP (#48696)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    the amber suppressor tRNACUA-Leu
  • Alt name
    synthetase AnapRS
  • Alt name
    Eukaryotic ribosomal release factor
  • Species
    H. sapiens (human)
  • Mutation
    mutant E.coli leucyl synthetase specific for Anap (U35 and A36 are mutated to C and U, respectively), E55D for eRF
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Addgene (#48696)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pANAP-P2A-eRF(E55D) was a gift from Justin Taraska (Addgene plasmid # 241290 ; http://n2t.net/addgene:241290 ; RRID:Addgene_241290)

  • For your References section:

    A PIP2-stabilized syntaxin-1a structure mapped with transition metal ion FRET and unnatural fluorescent amino acids at the plasma membrane. Obashi K, Strub MP, Taraska JW. Structure. 2025 Nov 24:S0969-2126(25)00388-0. doi: 10.1016/j.str.2025.10.001. 10.1016/j.str.2025.10.001 PubMed 41290003
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