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Addgene

pTol2_Ubi_N41_R307Q CNBD AcGFP
(Plasmid #241361)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241361 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pTol2pA2
  • Total vector size (bp) 11234
  • Vector type
    Tol2 zebrafish transgene expression; Gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ubi:CNBD_R307Q-AcGFP
  • Tag / Fusion Protein
    • AcGFP (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTol2_Ubi_N41_R307Q CNBD AcGFP was a gift from William Talbot (Addgene plasmid # 241361 ; http://n2t.net/addgene:241361 ; RRID:Addgene_241361)
  • For your References section:

    A cAMP Sensor Based on Ligand-Dependent Protein Stabilization. Sidoli M, Chen LC, Lu AJ, Wandless TJ, Talbot WS. ACS Chem Biol. 2022 Aug 19;17(8):2024-2030. doi: 10.1021/acschembio.2c00333. Epub 2022 Jul 15. 10.1021/acschembio.2c00333 PubMed 35839076