pTol2_Ubi_N41_R307Q CNBD AcGFP
(Plasmid
#241361)
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PurposepMPS55; ubiquitously expresses cAMP sensor(cAMP-insensitive mutant control); zebrafish transgene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241361 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepTol2pA2
- Total vector size (bp) 11234
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Vector typeTol2 zebrafish transgene expression; Gateway
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUbi:CNBD_R307Q-AcGFP
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Tag
/ Fusion Protein
- AcGFP (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTol2_Ubi_N41_R307Q CNBD AcGFP was a gift from William Talbot (Addgene plasmid # 241361 ; http://n2t.net/addgene:241361 ; RRID:Addgene_241361) -
For your References section:
A cAMP Sensor Based on Ligand-Dependent Protein Stabilization. Sidoli M, Chen LC, Lu AJ, Wandless TJ, Talbot WS. ACS Chem Biol. 2022 Aug 19;17(8):2024-2030. doi: 10.1021/acschembio.2c00333. Epub 2022 Jul 15. 10.1021/acschembio.2c00333 PubMed 35839076