pME_N41_CNBD_AcGFP
              
              
                (Plasmid
                
                #241362)
              
            
            
            
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            PurposepMPS15; middle entry clone holding N41_acgfp_cAMP sensor
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              Depositing Lab
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          Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepCR8/GTW
- Total vector size (bp) 3975
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              Vector typeGateway middle entry
Growth in Bacteria
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            Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberUnknown
Gene/Insert
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                Gene/Insert nameN41_CNBD-AcGFP
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        Tag
        / Fusion Protein
    - AcGFP (C terminal on insert)
 
Resource Information
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            Supplemental Documents
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms- Not Available to Industry
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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              For your Materials & Methods section: pME_N41_CNBD_AcGFP was a gift from William Talbot (Addgene plasmid # 241362 ; http://n2t.net/addgene:241362 ; RRID:Addgene_241362)
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                For your References section: A cAMP Sensor Based on Ligand-Dependent Protein Stabilization. Sidoli M, Chen LC, Lu AJ, Wandless TJ, Talbot WS. ACS Chem Biol. 2022 Aug 19;17(8):2024-2030. doi: 10.1021/acschembio.2c00333. Epub 2022 Jul 15. 10.1021/acschembio.2c00333 PubMed 35839076
