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pBabe-Tre-Rac1-P29S (FLARE)
(Plasmid #241371)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 241371 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBabe
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pak1, Rac1
  • Alt name
    FLARE
  • Alt name
    YPet-Pak1-T2A-P2A-mCerulean3-Rac1
  • Species
    H. sapiens (human)
  • Mutation
    P29S in Rac1
  • Entrez Gene
    PAK1 (a.k.a. IDDMSSD, PAKalpha, alpha-PAK, p65-PAK)
  • Entrez Gene
    RAC1 (a.k.a. MIG5, MRD48, Rac-1, TC-25, p21-Rac1)
  • Promoter TRE
  • Tags / Fusion Proteins
    • YPet (N terminal to Pak1)
    • mCerulean3 (N terminal to Rac1)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBabe-Tre-Rac1-P29S (FLARE) was a gift from Gaudenz Danuser (Addgene plasmid # 241371 ; http://n2t.net/addgene:241371 ; RRID:Addgene_241371)

  • For your References section:

    Morphological control of merlin-Rac antagonism in proliferation-promoting signaling. Weiss BG, Keth JM, Bhatt K, Doyal M, Hahn KM, Noh J, Isogai T, Danuser G. Sci Signal. 2025 May 20;18(887):eadk0922. doi: 10.1126/scisignal.adk0922. Epub 2025 May 20. 10.1126/scisignal.adk0922 PubMed 40392939
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