pMAL-pHluorin4
(Plasmid
#241868)
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PurposeExpresses pHluorin4 with N-terminal maltose binding protein in bacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241868 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepMAL
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Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 6200
- Total vector size (bp) 6378
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMBP-pHluorin4
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Insert Size (bp)1881
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySequence was synthesized based on the sequences of pHluorin2 (doi 10.4236/abb.2011.23021) and sfGFP (doi: 10.1038/nbt0906-1170d)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAL-pHluorin4 was a gift from Ian Thornell (Addgene plasmid # 241868 ; http://n2t.net/addgene:241868 ; RRID:Addgene_241868) -
For your References section:
The pH-dependent photophysical and spectral properties of pH-sensing green fluorescent proteins. Thornell IM. Physiol Rep. 2025 Oct;13(20):e70625. doi: 10.14814/phy2.70625. 10.14814/phy2.70625 PubMed 41108577