p131C-B10
(Plasmid
#241908)
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PurposeOptimised PCA biosensor produced sfGFP in response to hydroxyl-substituted benzoic acids, including protocatechuic acid (PCA)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 241908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepSEVA131
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDepositor recommends growing plasmids in BL21 E. coli cells.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepcaV, sfGFP
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SpeciesSynthetic
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Mutationnone
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p131C-B10 was a gift from Neil Dixon (Addgene plasmid # 241908 ; http://n2t.net/addgene:241908 ; RRID:Addgene_241908) -
For your References section:
Development of High-Performance Whole Cell Biosensors Aided by Statistical Modeling. Berepiki A, Kent R, Machado LFM, Dixon N. ACS Synth Biol. 2020 Mar 20;9(3):576-589. doi: 10.1021/acssynbio.9b00448. Epub 2020 Feb 17. 10.1021/acssynbio.9b00448 PubMed 32023410