pSEVA621-AzFRS
(Plasmid
#242002)
-
PurposeExpressed AzF tRNA synthetase for incorporation of p-azido-L-phenylalanine (pAzF)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 242002 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepSEVA621
-
Backbone manufacturerStandard European Vector Architecture
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Gentamicin, 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSEVA621-AzFRS was a gift from Wei Niu (Addgene plasmid # 242002 ; http://n2t.net/addgene:242002 ; RRID:Addgene_242002) -
For your References section:
Genetic Code Expansion in Pseudomonas putida KT2440. He X, Gao T, Chen Y, Liu K, Guo J, Niu W. ACS Synth Biol. 2022 Nov 18;11(11):3724-3732. doi: 10.1021/acssynbio.2c00325. Epub 2022 Oct 26. 10.1021/acssynbio.2c00325 PubMed 36287825