pMAL-c4X-MBP-ARID1A-IDR2
(Plasmid
#242216)
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PurposeExpression of MBP-tagged human ARID1A IDR2 (a.a. 1170-1610) in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242216 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepMAL-c4X
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameARID1A
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Alt nameBAF250a
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SpeciesH. sapiens (human)
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MutationARID1A IDR2 (a.a. 1170-1610)
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Entrez GeneARID1A (a.k.a. B120, BAF250, BAF250a, BM029, C1orf4, CSS2, ELD, MRD14, OSA1, P270, SMARCF1, hELD, hOSA1)
- Promoter tac
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Tag
/ Fusion Protein
- MBP (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Can be grown in BL21(DE3) cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAL-c4X-MBP-ARID1A-IDR2 was a gift from Courtney Hodges (Addgene plasmid # 242216 ; http://n2t.net/addgene:242216 ; RRID:Addgene_242216) -
For your References section:
beta-catenin functions as a molecular adapter for disordered cBAF interactions. Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, Cermakova K, Hodges HC. Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 10.1016/j.molcel.2025.06.026. 10.1016/j.molcel.2025.06.026 PubMed 40695292