pPB-CAG-CTNNB1-deltaN-HA
(Plasmid
#242218)
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PurposeExpression of human β-catenin ∆N terminal IDR (deletion of a.a. 2-133) using piggyBac transposition
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242218 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepPB
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Vector typeMammalian Expression ; piggyBac
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCTNNB1
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Alt nameβ-catenin
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SpeciesH. sapiens (human)
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MutationDeleted N terminal IDR from β-catenin (deletion of a.a. 2-133)
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Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
- Promoter CAG
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-CAG-CTNNB1-deltaN-HA was a gift from Courtney Hodges (Addgene plasmid # 242218 ; http://n2t.net/addgene:242218 ; RRID:Addgene_242218) -
For your References section:
beta-catenin functions as a molecular adapter for disordered cBAF interactions. Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, Cermakova K, Hodges HC. Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 10.1016/j.molcel.2025.06.026. 10.1016/j.molcel.2025.06.026 PubMed 40695292