pGEX-5X-1-GST-Armadillo
(Plasmid
#242225)
-
PurposeExpression of GST-tagged human β-catenin Armadillo repeats without IDRs (deletion of a.a. 2-133 and 672-781) in E. coli
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 242225 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
-
Vector backbonepGEX-5X-1
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCTNNB1
-
Alt nameβ-catenin
-
SpeciesH. sapiens (human)
-
MutationDeleted N terminal and C terminal IDRs from β-catenin (deletion of a.a. 2-133 and 672-781)
-
Entrez GeneCTNNB1 (a.k.a. CTNNB, EVR7, MRD19, NEDSDV, armadillo)
- Promoter tac
-
Tag
/ Fusion Protein
- GST (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Can be grown in BL21(DE3) cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGEX-5X-1-GST-Armadillo was a gift from Courtney Hodges (Addgene plasmid # 242225 ; http://n2t.net/addgene:242225 ; RRID:Addgene_242225) -
For your References section:
beta-catenin functions as a molecular adapter for disordered cBAF interactions. Chan YS, Gao Q, Robinson SA, Wang W, Filandrova R, Weinhold LM, Cabrera ML, Zhang M, Ambati CSR, Lerario AM, Putluri N, Kiseljak-Vassiliades K, Wierman ME, Habra MA, Hammer GD, Veverka V, Cermakova K, Hodges HC. Mol Cell. 2025 Jul 15:S1097-2765(25)00576-3. doi: 10.1016/j.molcel.2025.06.026. 10.1016/j.molcel.2025.06.026 PubMed 40695292