VEE-mScarlet3-IRES-EGFP-IRES-moxBFP
(Plasmid
#242407)
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PurposeNative (conventional) self-amplifying RNA (saRNA) control construct expresses moxBFP as visual marker of IRES functionality
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 242407 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backboneSimplicon Cloning Vector
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Backbone manufacturerMerck Millipore
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Vector typeMammalian Expression ; Self-amplifying RNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namemScarlet3
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SpeciesSynthetic
Gene/Insert 2
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Gene/Insert nameIRES-EGFP
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SpeciesSynthetic
Gene/Insert 3
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Gene/Insert nameIRES-moxBFP
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.09.24.614636 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VEE-mScarlet3-IRES-EGFP-IRES-moxBFP was a gift from Ewan St. John Smith (Addgene plasmid # 242407 ; http://n2t.net/addgene:242407 ; RRID:Addgene_242407) -
For your References section:
Cap-independent co-expression of dsRNA-sensing and NF-κB pathway inhibitors enables controllable self-amplifying RNA expression with reduced immunotoxicity. Lim TKY, Ritoux A, Paine LW, Ferguson L, Abdul T, Grundy LJ, Smith ESJ. bioRxiv 2024.09.24.614636 10.1101/2024.09.24.614636