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pPD0056_pIVTRup_dCas9-VP64
(Plasmid #242542)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242542 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pIVTRup
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-VP64
  • Species
    Other
  • Insert Size (bp)
    4413
  • Mutation
    D10A

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    10.1038/nature14136 (Konermann et al. Nature 2014)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Source insert Addgene ID 61425

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPD0056_pIVTRup_dCas9-VP64 was a gift from Christoph Bock & Paul Datlinger (Addgene plasmid # 242542 ; http://n2t.net/addgene:242542 ; RRID:Addgene_242542)

  • For your References section:

    Systematic discovery of CRISPR-boosted CAR T cell immunotherapies. Datlinger P, Pankevich EV, Arnold CD, Pranckevicius N, Lin J, Romanovskaia D, Schaefer M, Piras F, Orts AC, Nemc A, Biesaga PN, Chan M, Neuwirth T, Artemov AV, Li W, Ladstatter S, Krausgruber T, Bock C. Nature. 2025 Oct;646(8086):963-972. doi: 10.1038/s41586-025-09507-9. Epub 2025 Sep 24. 10.1038/s41586-025-09507-9 PubMed 40993398
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